Product Details
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Specificity
Detects N-terminal and C-terminal DYKDDDDK (FLAG®) epitope tagged proteins in Western blot.
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Source
Recombinant Monoclonal Rabbit IgG Clone # 1042E
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Purification
Protein A or G purified from cell culture supernatant
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Immunogen
DYKDDDDK epitope tag
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Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.
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Label
Unconjugated
Applications
Recommended
Concentration Sample
Western Blot 0.1 μg/mL See below
Simple Western 1 μg/mL See below
Flow Cytometry 0.25 μg/106 cells See below
Immunoprecipitation 1-4 μg/100 μg cell lysate
