培养基和添加剂
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RPMI-1640(GIBCO,货号31800022, 添加NaHCO3 1.5g/L, glucose 2.5g/L, Sodium Pyruvate 0.11g/L)和0.05mM β-巯基乙醇,90%;优质胎牛血清,10%。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。
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REFERENCE
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Cancer Res. 42: 1530-1536, 1982. PubMed: 6949641 J. Immunol. 131: 1882-1888, 1983. PubMed: 6619543 Cancer Res. 57: 3498-3504, 1997. PubMed: 9270019 Shanghai Immunnol 22(3):104-106,2002 Immunological Journal 18(3):278-280,2002 Acta Biochimica et Biophysica Sinica 35(12):1077-1082,2003 Prog. Biochem. Biophys. 30(6):940-944,2003 Acta Academae Medicinae Miltaris Tertiae 25(9):750-752,2003
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